• Identification of diagnostic marker proteins in cerebrospinal fluid for diagnosis of various types of meningitis, in particular tuberculous.
• Adenosine deaminase: A complementary tool in the early diagnosis of Tuberculous meningitis.
• Detection of Mycobacterium tuberculosis antigens and antibodies in CSF obtained from ventricle and lumbar puncture in patients of tuberculosis meningitis developing as complication.
• Identification of Biochemical and Immunological markers in serum of patients suffering from ischemic arterial stroke.
• A novel Multiplex PCR for simultaneous identification and discrimination of Mycobacterium tuberculosis, Mycobacterium avium complex and other non- tubercular Mycobacteria directly from clinical specimen.
• To study the diagnostic value of 30KD protein band in the serum of patients with extra CNS tuberculosis.
• Development and validation of the diagnostic kit for early diagnosis of tuberculous meningitis based on detection of 30KD/MTB antigen detection in CSF of suspected tuberculous meningitis by immunodiagnostic tests.
• Development of IS6110 PCR assay for diagnosis of CNS & Extra CNS Tuberculosis infections.
• Development of PCR based assay for diagnosis of HSV-1 and HSV-2 infection

ONGOING PROJECTS:

• Effects of certain herbal drugs on immunomodulation.
• Neuroprotective effect of certain Ayurvedic drugs after inducing Hypoxic Brain Ichemia.
• Cerebral Venous Sinus Thrombosis.
• Multiplex ELISA using different Antigens for diagnosis of pulmonary and extrapulmonaryTB
• Diagnosis of Chikungunya by ELISA based methods
• Proteomic approach for differential diagnosis of latent from active tuberculosis.
• Development of PCR based assay for diagnosis of CNS and extra CNS tuberculosis.
• Heterologous immunization approach using experimental animals to improve the efficacy of vaccines against TB.
• To study the role of heat shock protein in diagnosis and pathogenesis of tuberculosis.
• Identification of cytokine pattern secreted by monocytes (THP-1) cells after induction with Mycobacterium tuberculosis antigens.
• Identification of active component(s) of herbs possessing clot lytic activity through various analytical techniques.
• Invitro antibody production by using goat PBMCs
• Development of liquid and solid medium for isolation and identification of MTB
• Rapid diagnosis of tuberculosis Meningitis using Loop mediated isothermal amplification assay

UPCOMING PROJECTS:

• To identify the secreted components of neuroblastoma cell line mimicking as nerve growth factor for PC-12 cells.
• Diagnosis of Bacterial meningitis using molecular tools like PCR, Real time PCR, Loop mediated isothermal amplification assay
• Rapid diagnosis of tuberculosis meningitis using loop mediated isothermal amplification assay
• Development of animal and cell line model to study Brain edema in neurological complication.
• Usefulness of BacT/Alert in diagnosis of CNS bacterial infections
• Study on encephalitis like syndrome by development of immunological and molecular tools for the diagnosis of Human Herpes Virus CNS infection